2RL7
Crystal Structure cation-dependent mannose 6-phosphate receptor at pH 4.8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-04 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.840, 123.028, 53.092 |
| Unit cell angles | 90.00, 92.46, 90.00 |
Refinement procedure
| Resolution | 27.670 - 2.000 |
| Rwork | 0.211 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.046 | 0.035 | 0.099 |
| Number of reflections | 37928 | ||
| <I/σ(I)> | 28.1 | ||
| Completeness [%] | 86.0 | 97.5 | 50 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.8 | 277 | 0.05M cacodylate, 30% PEG5000MME, 0.2M ammonium sulfate, 0.09mM TritonX-100, pH 4.8, VAPOR DIFFUSION, temperature 277K |
