2RKM

STRUCTURE OF OPPA COMPLEXED WITH LYS-LYS

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.5
Synchrotron site	SRS
Beamline	PX9.5
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1996-08-04
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	110.470, 77.150, 71.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.167 *
Rwork	0.167
R-free	0.20200 *
RMSD bond length	0.006
RMSD bond angle	0.022
Data reduction software	DENZO
Data scaling software	SCALA
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.890
High resolution limit [Å]	1.800	1.800
Rmerge	0.072	0.219
Total number of observations	379533 *
Number of reflections	57099
<I/σ(I)>	7.6	3.4
Completeness [%]	92.9	98.9 *
Redundancy	6.6	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.5		7% PEG 4K, 50MM SODIUM ACETATE PH5.5, 1MM URANYL ACETATE AND 30MG/ML OPPA

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	drop	sodium acetate	50 (mM)
3	1	reservoir	PEG4000	6 (%)
4	1	reservoir	uranyl acetate	1 (mM)