2RKE
The Structure of rat cytosolic PEPCK in complex with sulfoacetate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-08-08 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.180, 119.031, 60.885 |
| Unit cell angles | 90.00, 108.84, 90.00 |
Refinement procedure
| Resolution | 29.090 - 1.800 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.24900 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.237 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
| Rmerge | 0.094 | 0.053 | 0.522 |
| Number of reflections | 53184 | ||
| <I/σ(I)> | 13.1 | ||
| Completeness [%] | 94.7 | 99.7 | 70.2 |
| Redundancy | 8.5 | 9.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 12-30% PEG 3350, 0.1M HEPES, 10 MM MNCL2, pH 7.4, temperature 298K, VAPOR DIFFUSION, HANGING DROP |
