2RK9
The crystal structure of a glyoxalase/bleomycin resistance protein/dioxygenase superfamily member from Vibrio splendidus 12B01
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.704, 76.954, 42.395 |
| Unit cell angles | 90.00, 92.00, 90.00 |
Refinement procedure
| Resolution | 40.680 - 1.600 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.22900 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.060 | 0.167 |
| Number of reflections | 32833 | |
| <I/σ(I)> | 16 | 5.5 |
| Completeness [%] | 94.6 | 65.7 |
| Redundancy | 6.7 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 294 | 0.2 M NaCl, 0.1 M Sodium acetate pH 4.6, 30% MPD, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
