2RK7

The Structure of rat cytosolic PEPCK in complex with oxalate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-06-19
Detector	RIGAKU RAXIS IV++
Spacegroup name	P 1 21 1
Unit cell lengths	64.011, 118.945, 86.506
Unit cell angles	90.00, 106.95, 90.00

Refinement procedure

Resolution	30.820 - 1.900
R-factor	0.207
Rwork	0.205
R-free	0.25600
RMSD bond length	0.009
RMSD bond angle	1.119
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	1.970
High resolution limit [Å]	1.900	4.090	1.900
Rmerge	0.130	0.062	0.680
Number of reflections	96505
<I/σ(I)>	7.9
Completeness [%]	99.2	98	96.3
Redundancy	5.5	5.9	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	15-30% PEG 3350, 0.1M HEPES,10 MM MNCL2, pH 7.4, temperature 293K, VAPOR DIFFUSION, HANGING DROP