2RJI
Malarial EBA-175 region VI crystallographic structure reveals a KIX-like binding interface
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX10.1 |
Synchrotron site | SRS |
Beamline | PX10.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.977 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 50.237, 65.591, 105.352 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.840 - 1.800 |
R-factor | 0.21003 |
Rwork | 0.208 |
R-free | 0.24114 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.247 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.880 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.040 | 0.357 |
Number of reflections | 16406 | |
<I/σ(I)> | 25.3 | 3.7 |
Completeness [%] | 99.3 | 99.7 |
Redundancy | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | 0.1M citric acid, 1M LiCl, 25-30% PEG 6K, pH4.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | 0.1M citric acid, 1M LiCl, 25-30% PEG 6K, pH4.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |