2RJB
Crystal structure of uncharacterized protein YdcJ (SF1787) from Shigella flexneri which includes domain DUF1338. Northeast Structural Genomics Consortium target SfR276
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-08-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.97900 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 192.209, 76.387, 159.052 |
| Unit cell angles | 90.00, 116.55, 90.00 |
Refinement procedure
| Resolution | 41.120 - 2.600 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.28100 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.072 | 0.228 |
| Number of reflections | 64525 | |
| <I/σ(I)> | 11.3 | 10 |
| Completeness [%] | 98.9 | 98.5 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH UNDER OIL | 7 | 277 | NH4H2PO4, 0.1M MOPS, 20% PEG 1000, pH 7.0, MICROBATCH UNDER OIL, temperature 277K |
