2RJ8
B-specific alpha-1,3-galactosyltransferase (GTB) +UDP+ H-antigen disaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-17 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 52.450, 149.600, 78.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.690 |
| R-factor | 0.199 |
| Rwork | 0.198 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.413 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.4SSI) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 19.790 | 1.750 |
| High resolution limit [Å] | 1.690 | 3.630 | 1.690 |
| Rmerge | 0.048 | 0.026 | 0.324 |
| Total number of observations | 18511 | 14640 | |
| Number of reflections | 34923 | ||
| <I/σ(I)> | 15.1 | 43.6 | 3.5 |
| Completeness [%] | 99.5 | 96.9 | 99.9 |
| Redundancy | 4.87 | 5.09 | 4.21 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7.5 | 298 | PEG4000 Glycerol MgCl NH2SO4, pH 7.5, hanging drop, temperature 298K |
