2RIV
Crystal structure of the reactive loop cleaved human Thyroxine Binding Globulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.5419 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 173.136, 42.585, 55.989 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.450 - 1.500 |
| R-factor | 0.19 |
| Rwork | 0.189 |
| R-free | 0.21401 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qmb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.310 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.500 | 1.539 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.041 | 0.222 |
| Number of reflections | 63059 | |
| <I/σ(I)> | 28.9 | 7.5 |
| Completeness [%] | 98.5 | 84.8 |
| Redundancy | 6.8 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.4 | 298 | 20% PEG 3350, 0.2M sodium sulphate, pH 7.4, EVAPORATION, temperature 298K |
