2RIJ

Crystal structure of a putative 2,3,4,5-tetrahydropyridine-2-carboxylate n-succinyltransferase (cj1605c, dapd) from campylobacter jejuni at 1.90 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2007-08-19
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97957, 0.95373, 0.97942
Spacegroup name	H 3 2
Unit cell lengths	135.093, 135.093, 213.742
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.540 - 1.900
R-factor	0.157
Rwork	0.156
R-free	0.18500
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.550
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.540	29.540	1.950
High resolution limit [Å]	1.900	8.500	1.900
Rmerge	0.124	0.011	0.011
Total number of observations		4835	32463
Number of reflections	59121
<I/σ(I)>	4.1	6.2	0.7
Completeness [%]	100.0	97.5	100
Redundancy	7.5	6.8	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	NANODROP, 1.0M Na Citrate, 0.1M Cacodylate pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K