2RI4
Crystal Structure determination of Goat Methemoglobin at 2.7 Angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-22 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5417 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.692, 68.325, 95.597 |
| Unit cell angles | 110.64, 91.90, 108.94 |
Refinement procedure
| Resolution | 22.500 - 2.700 |
| R-factor | 0.23371 |
| Rwork | 0.225 |
| R-free | 0.30815 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g08 Bovine carbonmonoxy hemoglobin |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.818 |
| Data reduction software | AUTOMAR |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.140 | |
| Number of reflections | 31524 | |
| <I/σ(I)> | 0.9 | |
| Completeness [%] | 90.4 | 93.4 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Unbuffered low-salt concentration using PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
