2RHF

D. radiodurans RecQ HRDC domain 3

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-26
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9999, 0.9797
Spacegroup name	P 41 21 2
Unit cell lengths	49.978, 49.978, 83.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.100
R-factor	0.17798
Rwork	0.177
R-free	0.19399
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.226
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	DM
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.600	1.160
High resolution limit [Å]	1.100	1.100
Number of reflections	40879
<I/σ(I)>		1.8
Completeness [%]	96.6	78.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	273	1.8 M Na-K Phosphate pH 4.0, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 273K