2RHB

Crystal structure of Nsp15-H234A mutant- Hexamer in asymmetric unit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-23
Detector	MAR CCD 165 mm
Spacegroup name	H 3
Unit cell lengths	305.757, 305.757, 88.740
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.800
R-factor	0.20043
Rwork	0.198
R-free	0.25953
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h85
RMSD bond length	0.006
RMSD bond angle	1.067
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	152.500	2.870
High resolution limit [Å]	2.800	2.800
Number of reflections	66521
Completeness [%]	91.9	78.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.9	289	at 10-15 mg/ml of protein in 0.05-0.1 M MgCl2, 3-5 % MPD and 0.1 M tri-sodium citrate dehydrate (pH 5.6-5.9). , VAPOR DIFFUSION, HANGING DROP, temperature 289K, pH 5.90