2RHB
Crystal structure of Nsp15-H234A mutant- Hexamer in asymmetric unit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-23 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | H 3 |
| Unit cell lengths | 305.757, 305.757, 88.740 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.20043 |
| Rwork | 0.198 |
| R-free | 0.25953 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h85 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.067 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 152.500 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 66521 | |
| Completeness [%] | 91.9 | 78.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.9 | 289 | at 10-15 mg/ml of protein in 0.05-0.1 M MgCl2, 3-5 % MPD and 0.1 M tri-sodium citrate dehydrate (pH 5.6-5.9). , VAPOR DIFFUSION, HANGING DROP, temperature 289K, pH 5.90 |
