2RGO
Structure of Alpha-Glycerophosphate Oxidase from Streptococcus sp.: A Template for the Mitochondrial Alpha-Glycerophosphate Dehydrogenase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9296 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 128.363, 106.785, 58.887 |
Unit cell angles | 90.00, 99.18, 90.00 |
Refinement procedure
Resolution | 12.000 - 2.400 |
R-factor | 0.23808 |
Rwork | 0.238 |
R-free | 0.24708 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rgh |
RMSD bond length | 0.013 |
RMSD bond angle | 1.675 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.890 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.298 | |
Number of reflections | 54822 | |
<I/σ(I)> | 9.9 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 1000, Tris, magnesium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |