2RFV

High resolution structure of L-methionine gamma-lyase from Citrobacter freundii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-04-01
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.843
Spacegroup name	I 2 2 2
Unit cell lengths	56.450, 122.800, 127.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.355
Rwork	0.151
R-free	0.17720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1y4i
RMSD bond length	0.008
RMSD bond angle	1.021
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.400
High resolution limit [Å]	1.350	1.350
Rmerge	0.034	0.340
Number of reflections	95252
<I/σ(I)>	20.15	3.54
Completeness [%]	95.6	84.5
Redundancy	3.8	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	303	35% MMEPEG 2000, 50MM TRIS-HCL,0.2MM PLP, 0.25% DTT, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 303K