2RF0
Crystal structure of human mixed lineage kinase MAP3K10 SH3 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00234 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 69.680, 69.680, 103.980 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.190 - 2.000 |
| R-factor | 0.19093 |
| Rwork | 0.189 |
| R-free | 0.23014 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 1OEB 1fmk |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.378 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0040) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.190 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.066 | 0.739 |
| Number of reflections | 19798 | |
| <I/σ(I)> | 21.1 | 4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.4 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2M Sodium malonate, 0.1M Bis-tris-propane pH 8.5, 20.0% PEG 3350, 10.0% Ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
