2REX
Crystal structure of the effector domain of PLXNB1 bound with Rnd1 GTPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-24 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.91790 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.136, 71.653, 101.885 |
| Unit cell angles | 90.00, 128.36, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.216 |
| Rwork | 0.215 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 2CLS 2r2o |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.448 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.060 | 0.053 | 0.231 |
| Number of reflections | 36932 | ||
| <I/σ(I)> | 9.1 | ||
| Completeness [%] | 97.3 | 97.8 | 84.8 |
| Redundancy | 3.7 | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20% PEG 3350, 0.2M Calcium chloride, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
