2RE8
Glutaminyl-tRNA synthetase mutant C229R with bound analog 5'-O-[N-(L-GLUTAMYL)-SULFAMOYL]ADENOSINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-02 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 237.142, 92.907, 115.512 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 118.600 - 2.600 |
| Rwork | 0.223 |
| R-free | 0.25400 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.237 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 118.678 | 118.680 | 2.360 |
| High resolution limit [Å] | 2.300 | 10.290 | 2.300 |
| Rmerge | 0.125 | 0.049 | 0.808 |
| Total number of observations | 2840 | 9772 | |
| Number of reflections | 52739 | ||
| <I/σ(I)> | 4.4 | 11.2 | 0.8 |
| Completeness [%] | 93.2 | 94.9 | 77.6 |
| Redundancy | 3.8 | 4.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | Ammonium Sulfate, pH 7.5, VAPOR DIFFUSION, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Ammonium Sulfate | ||
| 2 | 1 | 1 | H2O | ||
| 3 | 1 | 2 | Ammonium Sulfate | ||
| 4 | 1 | 2 | H2O |
