2RE3
CRYSTAL STRUCTURE OF a DUF1285 family protein (SPO_0140) FROM SILICIBACTER POMEROYI DSS-3 AT 2.50 A RESOLUTION
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-13 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837, 0.97908, 0.97935 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 75.370, 75.370, 182.687 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.488 - 2.500 |
| R-factor | 0.217 |
| Rwork | 0.215 |
| R-free | 0.25800 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.557 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.488 | 29.488 | 2.560 |
| High resolution limit [Å] | 2.500 | 11.180 | 2.500 |
| Rmerge | 0.129 | 0.042 | 0.760 |
| Total number of observations | 1501 | 10334 | |
| Number of reflections | 19038 | ||
| <I/σ(I)> | 4.9 | 12.9 | 1 |
| Completeness [%] | 99.9 | 94.5 | 100 |
| Redundancy | 7.4 | 5.8 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | NANODROP, 20.0% Ethanol, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
