2RCY
Crystal structure of Plasmodium falciparum pyrroline carboxylate reductase (MAL13P1.284) with NADP bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97937 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 160.207, 113.741, 82.651 |
Unit cell angles | 90.00, 105.71, 90.00 |
Refinement procedure
Resolution | 46.270 - 2.300 |
R-factor | 0.247 |
Rwork | 0.245 |
R-free | 0.28900 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.941 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.270 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.142 | 0.496 |
Number of reflections | 57815 | |
<I/σ(I)> | 7.3 | 1.1 |
Completeness [%] | 89.7 | 53.6 |
Redundancy | 2.1 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 30% PEG MME550, 50 mM MgCl2, 0.1 M Hepes pH 7.5, 5 mM NADP, VAPOR DIFFUSION, HANGING DROP, temperature 291K |