2RCQ
Crystal structure of human apo Cellular Retinol Binding Protein II (CRBP-II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-12 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.518, 49.834, 74.398 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.200 |
| R-factor | 0.197 |
| Rwork | 0.196 |
| R-free | 0.21700 |
| Starting model (for MR) | 1opa |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.044 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.000 | 1.230 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.047 | 0.187 |
| Number of reflections | 40046 | |
| <I/σ(I)> | 18.9 | 8 |
| Completeness [%] | 96.0 | 95.4 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1 M sodium citrate, 0.2 M sodium/potassium tartrate, 1.4 M ammonium sulphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
