2RBM
Crystal structure of Staphylococcal nuclease variant Delta+PHS I72K at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2007-08-06 |
Detector | BRUKER APEX II |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 32.229, 60.603, 72.579 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.520 - 1.900 |
R-factor | 0.17981 |
Rwork | 0.173 |
R-free | 0.23795 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1TQO.PDB where all waters removed all Bs=20.0 A^2 and I72A and E92A |
RMSD bond length | 0.016 |
RMSD bond angle | 1.950 |
Data reduction software | SAINT |
Data scaling software | SAINT (V7.34A) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.520 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 11779 | |
<I/σ(I)> | 37.25 | 6.13 |
Completeness [%] | 100.0 | 100 |
Redundancy | 18.9 | 8.75 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 33% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |