2RBG

Crystal structure of hypothetical protein(ST0493) from sulfolobus tokodaii

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-16
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97899, 0.9, 0.97931
Spacegroup name	P 1 21 1
Unit cell lengths	39.444, 65.184, 49.604
Unit cell angles	90.00, 98.19, 90.00

Refinement procedure

Resolution	33.490 - 1.750
R-factor	0.173
Rwork	0.173
R-free	0.19600
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.810
High resolution limit [Å]	1.750	1.750
Rmerge	0.059	0.143
Number of reflections	25105
Completeness [%]	99.6	96.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.5	293	30% PEG 4K, 0.2M Ammonium Sulfate, pH 7.5, MICROBATCH, temperature 293K