2RBB

Crystal structure of a glyoxalase/bleomycin resistance protein/dioxygenase family enzyme from Burkholderia phytofirmans PsJN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-09-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97930
Spacegroup name	P 42 21 2
Unit cell lengths	83.230, 83.230, 99.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.930 - 1.820
R-factor	0.239
Rwork	0.239
R-free	0.25900
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.890
High resolution limit [Å]	1.820	1.820
Rmerge	0.073
Number of reflections	32129
<I/σ(I)>	10.4	2.3
Completeness [%]	100.0	99.9
Redundancy	27.1	17.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	2M Sodium citrate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K