2RAP
THE SMALL G PROTEIN RAP2A IN COMPLEX WITH GTP
Experimental procedure
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW21B |
Synchrotron site | LURE |
Beamline | DW21B |
Temperature [K] | 278 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 61 |
Unit cell lengths | 64.500, 64.500, 84.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 2.600 |
R-factor | 0.206 |
Rwork | 0.206 |
R-free | 0.28100 |
Structure solution method | DIFFERENCE FOURIER MAPS |
RMSD bond length | 0.010 |
RMSD bond angle | 2.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.500 |
Rmerge | 0.065 |
Total number of observations | 46223 * |
Number of reflections | 8828 |
Completeness [%] | 98.5 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 | 25% PEG 8000, 100MM LISO4, 100MM TRIS PH 8., pH 8.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 25 (%) | |
2 | 1 | reservoir | 100 (mM) | ||
3 | 1 | reservoir | Tris-HCl | 100 (mM) |