2RAF

Crystal structure of putative dinucleotide-binding oxidoreductase (NP_786167.1) from Lactobacillus plantarum at 1.60 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-07-25
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97886, 0.97917
Spacegroup name	C 2 2 21
Unit cell lengths	53.330, 152.570, 140.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.540 - 1.600
R-factor	0.153
Rwork	0.151
R-free	0.18600
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.751
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	REFMAC (5.3.0040)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.540	29.540	1.660
High resolution limit [Å]	1.600	3.440	1.600
Rmerge	0.053	0.020	0.368
Number of reflections	75349	14311	14986
<I/σ(I)>	10.8	32.7	2.2
Completeness [%]	99.2	97.4	98.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	NANODROP, 20.0% PEG 3350, 0.2M Ammonium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 293K