2RAE
Crystal structure of a TetR/AcrR family transcriptional regulator from Rhodococcus sp. RHA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 65.107, 65.107, 106.627 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.560 - 2.200 |
| R-factor | 0.2339 |
| Rwork | 0.233 |
| R-free | 0.25257 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.370 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.560 | 2.257 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.084 | 0.580 |
| Number of reflections | 11548 | |
| <I/σ(I)> | 49.3 | 1.85 |
| Completeness [%] | 99.2 | 97.6 |
| Redundancy | 16.8 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 20% PEG 3350, 0.2M K Citrate, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
