2R94

Crystal Structure of KD(P)GA from T.tenax

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-20
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.98
Spacegroup name	P 43 21 2
Unit cell lengths	149.760, 149.760, 173.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.950 - 2.200
R-factor	0.188
Rwork	0.185
R-free	0.22500
Starting model (for MR)	1w3n
RMSD bond length	0.019
RMSD bond angle	1.801
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.950	2.500
High resolution limit [Å]	2.200	2.200
Rmerge	0.086	0.230
Number of reflections	100096
<I/σ(I)>	14.48	5.02
Completeness [%]	100.0	100
Redundancy	9.8	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	298	HEPES/KOH, pH 7, vapor diffusion, temperature 298K