2R94
Crystal Structure of KD(P)GA from T.tenax
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 149.760, 149.760, 173.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.950 - 2.200 |
| R-factor | 0.188 |
| Rwork | 0.185 |
| R-free | 0.22500 |
| Starting model (for MR) | 1w3n |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.801 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.950 | 2.500 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.086 | 0.230 |
| Number of reflections | 100096 | |
| <I/σ(I)> | 14.48 | 5.02 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.8 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | HEPES/KOH, pH 7, vapor diffusion, temperature 298K |
