2R92
Elongation complex of RNA polymerase II with artificial RdRP scaffold
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 222.675, 393.853, 283.551 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.800 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.470 |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.940 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Number of reflections | 122098 | |
| <I/σ(I)> | 21.38 | 6.77 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.5 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 295 | 5% (w/v) PEG 6000, 200 mM ammonium acetate, 150 mM magnesium acetate, 50 mM Hepes pH 7.0, 5 mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K, pH 7.00 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 6000 | ||
| 10 | 1 | 2 | TCEP | ||
| 2 | 1 | 1 | ammonium acetate | ||
| 3 | 1 | 1 | magnesium acetate | ||
| 4 | 1 | 1 | Hepes | ||
| 5 | 1 | 1 | TCEP | ||
| 6 | 1 | 2 | PEG 6000 | ||
| 7 | 1 | 2 | ammonium acetate | ||
| 8 | 1 | 2 | magnesium acetate | ||
| 9 | 1 | 2 | Hepes |
