2R8P
Transketolase from E. coli in complex with substrate D-fructose-6-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-12-07 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.8423 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 90.214, 101.863, 133.345 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.870 - 1.650 |
R-factor | 0.156 |
Rwork | 0.156 |
R-free | 0.18000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.347 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.470 | 99.000 | 1.670 |
High resolution limit [Å] | 1.650 | 5.130 | 1.650 |
Rmerge | 0.082 | 0.034 | 0.518 |
Number of reflections | 147102 | ||
<I/σ(I)> | 10.6 | ||
Completeness [%] | 99.5 | 99.5 | 98 |
Redundancy | 7.2 | 7.2 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 281 | PEG 6000, glycerol, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 281K |