2R7E

Crystal Structure Analysis of Coagulation Factor VIII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9794
Spacegroup name	P 41 21 2
Unit cell lengths	134.569, 134.569, 359.496
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.370 - 3.700
R-factor	0.279
Rwork	0.279
R-free	0.34700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2j5w and 1d7p
RMSD bond length	0.010
RMSD bond angle	1.800
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	150.000	3.660
High resolution limit [Å]	3.600	3.600
Rmerge	0.122	0.857
Number of reflections	36577
<I/σ(I)>	18	0.7
Completeness [%]	92.9	55
Redundancy	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	8-10% PEG 8000, 0.1M Imidazole, 100-300 mM NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	8-10% PEG 8000, 0.1M Imidazole, 100-300 mM NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K