2R74
Crystal Structure of the Possum Milk Whey Lipocalin Trichosurin at pH 4.6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-02-12 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5419 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.850, 44.580, 70.410 |
| Unit cell angles | 90.00, 101.50, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.900 |
| Rwork | 0.214 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2A2U rat major urinary protein alpha2u-globulin |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.283 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.043 | 0.190 |
| Number of reflections | 26645 | |
| <I/σ(I)> | 17.6 | 6.6 |
| Completeness [%] | 95.1 | 88.9 |
| Redundancy | 4.03 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 8% 2-propanol, 200mM CaCl2, 100mM NaOAc, pH 4.6, vapor diffusion, hanging drop, temperature 291K |
