2R6F

Crystal Structure of Bacillus stearothermophilus UvrA

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Wavelength(s)	0.97925, 0.97941, 0.96403
Spacegroup name	P 1 21 1
Unit cell lengths	102.713, 94.720, 130.481
Unit cell angles	90.00, 108.80, 90.00

Refinement procedure

Resolution	50.000 - 3.200
R-factor	0.257
Rwork	0.253
R-free	0.29200
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.247
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.200	3.200
Rmerge	0.079	0.305
Number of reflections	38657
<I/σ(I)>	13.4
Completeness [%]	98.3	85.9
Redundancy	6.9	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	13%PEG2000MME, 50 mM Na-HEPES pH 7.0, 500 mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	7	295	13%PEG2000MME, 50 mM Na-HEPES pH 7.0, 500 mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	7	295	13%PEG2000MME, 50 mM Na-HEPES pH 7.0, 500 mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 295K