2R5A
Crystal Structure of the two MBT repeats from Sex-Comb on Midleg (SCM) in complex with methyl lysine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | .934 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 83.030, 83.030, 75.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.420 - 2.300 |
| R-factor | 0.17473 |
| Rwork | 0.173 |
| R-free | 0.22147 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.303 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.420 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.072 | 0.482 |
| Number of reflections | 12304 | |
| <I/σ(I)> | 6.6 | 1.7 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 15.3 | 16.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.3 | 293 | 15% (w/v) PEG3350, 100 mM calcium acetate and 3% ethanol, pH 7.3, VAPOR DIFFUSION, temperature 293K |
