2R53
Crystal structure analysis of Bone Morphogenetic Protein-6 variant B2 (B2-BMP-6)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-03 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9183 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 99.780, 99.780, 86.760 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.261 |
| Rwork | 0.259 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bmp |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.475 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 2.250 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 28518 | |
| <I/σ(I)> | 13.5 | 4.1 |
| Completeness [%] | 95.2 | 95.7 |
| Redundancy | 8.1 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 25% 2-Methyl-2,4-pentandiol, 0.1M sodium citrate pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
