2R4O

Crystal structure of the long-chain fatty acid transporter FadL mutant delta NPA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-17
Detector	ADSC QUANTUM 210
Spacegroup name	P 21 21 21
Unit cell lengths	63.050, 146.618, 151.227
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 3.600
R-factor	0.243
Rwork	0.243
R-free	0.31400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t16
RMSD bond length	0.009
RMSD bond angle	1.550
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.540	3.730
High resolution limit [Å]	3.600	3.600
Rmerge	0.287	0.593
Number of reflections	16885
<I/σ(I)>	3.1	2.2
Completeness [%]	98.7	97
Redundancy	4.4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5	295	35% PEG 2000, 0.05M magnesium acetate, 0.05M cacodylate, pH 5.50, VAPOR DIFFUSION, HANGING DROP, temperature 295K