2R42
The Biochemical and Structural Basis for feedback Inhibition of Mevalonate Kinase and Isoprenoid Metabolism
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-06-15 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 77.990, 118.430, 42.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.030 - 2.400 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.27200 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.030 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 13785 | |
| <I/σ(I)> | 12.23 | 1.8 |
| Completeness [%] | 84.4 | 48.3 |
| Redundancy | 2.5 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 277 | HEPES buffer, PEG5000 MME, MgCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 7.50 |
