2R3L

Crystal Structure of Cyclin-Dependent Kinase 2 with inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-BM
Synchrotron site	APS
Beamline	17-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2002-10-01
Detector	MARRESEARCH
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	53.650, 72.580, 72.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.800 - 1.650
R-factor	0.195
Rwork	0.194
R-free	0.22600
Structure solution method	FOURIER SYNTHESIS
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	BUSTER-TNT

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.660
High resolution limit [Å]	1.650	5.640	1.650
Rmerge	0.045	0.019	0.319
Number of reflections	32217
<I/σ(I)>	15.6
Completeness [%]	92.7	87.2	60.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.4	298	50 mM Na-HEPES pH 7.4, 50 mM Ammonium Acetate, 8% PEG 4000, 4% Glycerol, 1 mM TCEP, vapor diffusion, temperature 298K