2R39
Crystal structure of FixG-related protein from Vibrio parahaemolyticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-04 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97891 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 74.567, 74.567, 49.360 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.020 - 2.020 |
| R-factor | 0.20513 |
| Rwork | 0.203 |
| R-free | 0.23815 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.326 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.069 | 0.273 |
| Number of reflections | 10403 | |
| <I/σ(I)> | 50.53 | 7.25 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 10.1 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | HEPES pH 7.5, 30% PEG 400, NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
