2R29
Neutralization of dengue virus by a serotype cross-reactive antibody elucidated by cryoelectron microscopy and x-ray crystallography
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-29 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.120, 58.660, 78.190 |
| Unit cell angles | 90.00, 114.41, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| Rwork | 0.259 |
| R-free | 0.32100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 6.030 | 2.800 |
| Rmerge | 0.123 | 0.080 | 0.470 |
| Number of reflections | 15759 | ||
| <I/σ(I)> | 9.4 | ||
| Completeness [%] | 90.2 | 100 | 39.2 |
| Redundancy | 3.4 | 3.7 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 5.4 | 298 | 11-13% PEG 2000, pH 5.4, hanging drop, temperature 298K |
