2R21
Structure of the RNA brominated tridecamer r(GCGUU-5BUGAAACGC) at 1.6 A (BrMn)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 80 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-05-04 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.919487,0.919704,0.88558,0.979776 |
| Spacegroup name | P 1 |
| Unit cell lengths | 25.250, 25.650, 29.720 |
| Unit cell angles | 98.13, 106.15, 100.83 |
Refinement procedure
| Resolution | 20.000 - 1.590 |
| Rwork | 0.169 |
| R-free | 0.19100 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.893 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.680 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.117 | 0.230 |
| Number of reflections | 8713 | |
| <I/σ(I)> | 4.8 | 2.6 |
| Completeness [%] | 94.4 | 84.9 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 0.5mM MnCl2, 100mM NaCl, 30% MPD, 50mM Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | Tris | ||
| 4 | 1 | 1 | MnCl2 | ||
| 5 | 1 | 2 | MPD | ||
| 6 | 1 | 2 | NaCl | ||
| 7 | 1 | 2 | Tris | ||
| 8 | 1 | 2 | MnCl2 |
