2R20
Structure of the RNA brominated tridecamer r(GCGUU-5BUGAAACGC) at 1.3 A (Br2)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 80 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-05-04 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.919487,0.919704,0.88558,0.979776 |
| Spacegroup name | P 1 |
| Unit cell lengths | 25.280, 25.800, 29.990 |
| Unit cell angles | 98.62, 105.58, 100.92 |
Refinement procedure
| Resolution | 20.000 - 1.300 |
| Rwork | 0.181 |
| R-free | 0.19500 |
| Structure solution method | MAD |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.450 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 13060 | |
| <I/σ(I)> | 9.1 | 8.6 |
| Completeness [%] | 91.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 5 mM MgCl2, 5 mM MnCl2, 100 mM NaCl, 30 % MPD, 50 mM tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 10 | 1 | 2 | MnCl2 | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | tris | ||
| 4 | 1 | 1 | MgCl2 | ||
| 5 | 1 | 1 | MnCl2 | ||
| 6 | 1 | 2 | MPD | ||
| 7 | 1 | 2 | NaCl | ||
| 8 | 1 | 2 | tris | ||
| 9 | 1 | 2 | MgCl2 |
