2R1U
DJ-1 activation by catechol quinone modification
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-22 |
| Spacegroup name | P 31 |
| Unit cell lengths | 75.688, 75.688, 75.045 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.680 - 1.500 |
| Rwork | 0.185 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.316 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.040 | 0.040 |
| Number of reflections | 71845 | |
| <I/σ(I)> | 40 | 40 |
| Completeness [%] | 93.3 | 100 |
| Redundancy | 6.5 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 29%PEG4K, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
