2R1A
Crystal structure of the periplasmic lipopolysaccharide transport protein LptA (YhbN), trigonal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.977173 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 146.210, 146.210, 186.810 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.980 - 3.260 |
| R-factor | 0.3011 |
| Rwork | 0.298 |
| R-free | 0.36086 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r19 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.035 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0065) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.370 |
| High resolution limit [Å] | 3.250 | 7.000 | 3.250 |
| Rmerge | 0.127 | 0.050 | 0.614 |
| Number of reflections | 30606 | ||
| <I/σ(I)> | 8.3 | ||
| Completeness [%] | 97.3 | 98.4 | 81.8 |
| Redundancy | 8.9 | 9.5 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | PEG 3350, Glycerol, LPS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
