2R19

Crystal structure of the periplasmic lipopolysaccharide transport protein LptA (YhbN), orthorhombic form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-16
Detector	ADSC QUANTUM 210
Wavelength(s)	1.10000
Spacegroup name	P 21 21 21
Unit cell lengths	47.910, 58.410, 149.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.030 - 2.160
R-factor	0.219
Rwork	0.217
R-free	0.27100
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.611
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.230
High resolution limit [Å]	2.150	4.630	2.150
Rmerge	0.116	0.031	0.739
Number of reflections	13258
<I/σ(I)>	6.5
Completeness [%]	99.6	97.6	100
Redundancy	6.4	6.6	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	PEG 3350, Glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	PEG 3350, Glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K