2R16
Crystal Structure of bovine neurexin 1 alpha LNS/LG domain 4 (with no splice insert)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2006-08-16 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.96112 |
| Spacegroup name | P 63 |
| Unit cell lengths | 90.322, 90.322, 39.624 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.700 - 1.040 |
| R-factor | 0.14839 |
| Rwork | 0.146 |
| R-free | 0.16518 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c4r |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.906 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.700 | 1.080 |
| High resolution limit [Å] | 1.040 | 1.040 |
| Rmerge | 0.070 | 0.296 |
| Number of reflections | 82197 | |
| <I/σ(I)> | 37.9 | 6.2 |
| Completeness [%] | 92.9 | 60.5 |
| Redundancy | 12.6 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 12.5% isopropanol, 0.1 M CHES pH 9.0, 5 mM CaCl2, , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
