2R0X
Crystal structure of a putative flavin reductase (ycdh, hs_1225) from haemophilus somnus 129pt at 1.06 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-01 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91837, 0.97925, 0.97898 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 72.020, 38.850, 48.440 |
Unit cell angles | 90.00, 115.63, 90.00 |
Refinement procedure
Resolution | 23.402 - 1.060 |
R-factor | 0.143 |
Rwork | 0.141 |
R-free | 0.17000 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.667 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 23.402 | 23.402 | 1.100 |
High resolution limit [Å] | 1.060 | 1.810 | 1.060 |
Rmerge | 0.070 | 0.044 | 0.678 |
Number of reflections | 54635 | ||
<I/σ(I)> | 6.34 | 14.3 | 1.34 |
Completeness [%] | 98.9 | 98.3 | 97.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | NANODROP, 2.0M Ammonium sulfate, 0.1M Sodium acetate pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |