2R0T
Crystal structure of GDP-4-keto-6-deoxymannose-3-dehydratase with a trapped PLP-glutamate geminal diamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2007-06-20 |
| Detector | BRUKER PROTEUM |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.908, 72.905, 86.959 |
| Unit cell angles | 90.00, 107.94, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gmu |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | EPMR |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 60929 | |
| <I/σ(I)> | 9.87 | 1.55 |
| Completeness [%] | 92.8 | 83.3 |
| Redundancy | 2.9 | 1.42 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 298 | 24% PEG 3400, 100mM MES, 600mM KCl, 2mM PLP, 2mM 2-oxoglutarate, pH 6.0, batch, temperature 298K |
