2R0O

Crystal structure of the actin-binding domain of human alpha-actinin-4 mutant(K255E)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	200
Detector technology	CCD
Collection date	2007-04-21
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	61.697, 61.533, 174.936
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.370 - 2.200
R-factor	0.17427
Rwork	0.171
R-free	0.22388
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2eyi
RMSD bond length	0.014
RMSD bond angle	1.398
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.400	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.088	0.283
Number of reflections	25757
<I/σ(I)>	33.8	6.6
Completeness [%]	100.0	100
Redundancy	5.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	277.15	100 mM Imidazole, 50 mM NaCl, 1 mM EDTA, 5 % (v/v) glycerol, and 21 % (w/v) polyethylene glycol 5000 mono-methyl-ether, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K