2R0J
Crystal structure of the putative ubiquitin conjugating enzyme, PFE1350c, from Plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ DW |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-06-12 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 73.747, 73.747, 79.112 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.990 - 1.850 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qcq |
| RMSD bond length | 0.033 |
| RMSD bond angle | 2.313 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.047 | 0.567 |
| Number of reflections | 19224 | |
| <I/σ(I)> | 20.6 | 1.6 |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 13.8 | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 3.5M Sodium formate, 0.1M Bis-Tris pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
